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mirxplore ™ microarray kits  (Miltenyi Biotec)


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    Miltenyi Biotec mirxplore ™ microarray kits
    Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same <t>microarray.</t> (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.
    Mirxplore ™ Microarray Kits, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mirxplore+microarray/pmc03303677-14-3-8?v=Miltenyi+Biotec
    Average 90 stars, based on 1 article reviews
    mirxplore ™ microarray kits - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "How Can Microarrays Unlock Asthma?"

    Article Title: How Can Microarrays Unlock Asthma?

    Journal: Journal of Allergy

    doi: 10.1155/2012/241314

    Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.
    Figure Legend Snippet: Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.

    Techniques Used: Expressing, Derivative Assay, Labeling, Microarray, Amplification, Binding Assay, Staining, Hybridization, Sequencing

    Databases used in Affymetrix microarray annotation.
    Figure Legend Snippet: Databases used in Affymetrix microarray annotation.

    Techniques Used: Microarray, Sequencing, Construct, Expressing

    List of a number companies currently providing miRNA microarray technology.
    Figure Legend Snippet: List of a number companies currently providing miRNA microarray technology.

    Techniques Used: Microarray

    The GEO accession number for microarray studies conducted on asthma.
    Figure Legend Snippet: The GEO accession number for microarray studies conducted on asthma.

    Techniques Used: Microarray, Expressing, Clone Assay, Infection, Functional Assay, Sequencing, Synthesized



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    Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same <t>microarray.</t> (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.
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    Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.

    Journal: Journal of Allergy

    Article Title: How Can Microarrays Unlock Asthma?

    doi: 10.1155/2012/241314

    Figure Lengend Snippet: Overview of the production and use of expression microarrays. 3′ Expression arrays use synthetically derived oligo probes with design based on mRNA Databases (RefSeq mRNAs, GenBank mRNAs, and ESTs from dbEST) or cDNA derived from bacterial libraries (see ). Sample mRNA can be labeled using two methods (a) Cy3/Cy5 labeling: sample mRNA is reverse transcribed into cDNA and Cy3 is added to one sample and Cy5 to another. Both labeled samples are hybridized to the same microarray. (b) 3′ IVT array: sample mRNA is reverse transcribed to cDNA using oligo(dT) primers, to provide a template for transcription. Using biotin-conjugated nucleotides, the template cDNA is then converted to amplified RNA (aRNA). The biotin-labeled aRNA samples are then fragmented and hybridized onto 3′ expression arrays. A biotin binding fluorescent stain is added to the microarray after hybridization. (c) Affymetrix HuExon 1.0 ST: sample mRNA is reverse transcribed to cDNA using random primers, to provide a template for transcription. The resulting RNA is then reverse transcribed in the presence of dUTPs which are incorporated occasionally into the cDNA sequence instead of dTTP. An enzyme is then used to cleave the cDNA at the site of dUTP incorporation and fragments are terminally labeled before hybridization onto the array. The microarray is then washed and stained after hybridization.

    Article Snippet: Miltenyi Biotec , miRXplore ™ Microarray Kits , http://www.miltenyibiotec.com/.

    Techniques: Expressing, Derivative Assay, Labeling, Microarray, Amplification, Binding Assay, Staining, Hybridization, Sequencing

    Databases used in Affymetrix microarray annotation.

    Journal: Journal of Allergy

    Article Title: How Can Microarrays Unlock Asthma?

    doi: 10.1155/2012/241314

    Figure Lengend Snippet: Databases used in Affymetrix microarray annotation.

    Article Snippet: Miltenyi Biotec , miRXplore ™ Microarray Kits , http://www.miltenyibiotec.com/.

    Techniques: Microarray, Sequencing, Construct, Expressing

    List of a number companies currently providing miRNA microarray technology.

    Journal: Journal of Allergy

    Article Title: How Can Microarrays Unlock Asthma?

    doi: 10.1155/2012/241314

    Figure Lengend Snippet: List of a number companies currently providing miRNA microarray technology.

    Article Snippet: Miltenyi Biotec , miRXplore ™ Microarray Kits , http://www.miltenyibiotec.com/.

    Techniques: Microarray

    The GEO accession number for microarray studies conducted on asthma.

    Journal: Journal of Allergy

    Article Title: How Can Microarrays Unlock Asthma?

    doi: 10.1155/2012/241314

    Figure Lengend Snippet: The GEO accession number for microarray studies conducted on asthma.

    Article Snippet: Miltenyi Biotec , miRXplore ™ Microarray Kits , http://www.miltenyibiotec.com/.

    Techniques: Microarray, Expressing, Clone Assay, Infection, Functional Assay, Sequencing, Synthesized

    Platform description.

    Journal: PLoS ONE

    Article Title: Comparison of Microarray Platforms for Measuring Differential MicroRNA Expression in Paired Normal/Cancer Colon Tissues

    doi: 10.1371/journal.pone.0045105

    Figure Lengend Snippet: Platform description.

    Article Snippet: RNA aliquots from these samples were hybridized on four microarray platforms: Agilent SurePrint G3 human miRNA Microarray, Exiqon miRCURY LNA microRNA Array, Illumina Human_v2 microRNA expression Beadchips, and Miltenyi miRXplore Microarray.

    Techniques: Microarray, Labeling, Ligation

    (A) Pathway enrichment analysis of anti-correlated predicted target genes of differentially expressed miRNAs according to each microarray platform. (B) Network between the top 8 differentially expressed miRNAs and their anti-correlated target genes. The 250 top interactions were used to generate the network using MAGIA tool.

    Journal: PLoS ONE

    Article Title: Comparison of Microarray Platforms for Measuring Differential MicroRNA Expression in Paired Normal/Cancer Colon Tissues

    doi: 10.1371/journal.pone.0045105

    Figure Lengend Snippet: (A) Pathway enrichment analysis of anti-correlated predicted target genes of differentially expressed miRNAs according to each microarray platform. (B) Network between the top 8 differentially expressed miRNAs and their anti-correlated target genes. The 250 top interactions were used to generate the network using MAGIA tool.

    Article Snippet: RNA aliquots from these samples were hybridized on four microarray platforms: Agilent SurePrint G3 human miRNA Microarray, Exiqon miRCURY LNA microRNA Array, Illumina Human_v2 microRNA expression Beadchips, and Miltenyi miRXplore Microarray.

    Techniques: Microarray